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Mobile robot navigation is a critical aspect of robotics, with applications spanning from service robots to industrial automation. However, navigating in complex and dynamic environments poses many challenges, such as avoiding obstacles, making decisions in real-time, and adapting to new situations. Reinforcement Learning (RL) has emerged as a promising approach to enable robots to learn navigation policies from their interactions with the environment. However, application of RL methods to real-world tasks such as mobile robot navigation, and evaluating their performance under various training–testing settings has not been sufficiently researched. In this paper, we have designed an evaluation framework that investigates the RL algorithm’s generalization capability in regard to unseen scenarios in terms of learning convergence and success rates by transferring learned policies in simulation to physical environments. To achieve this, we designed a simulated environment in Gazebo for training the robot over a high number of episodes. The training environment closely mimics the typical indoor scenarios that a mobile robot can encounter, replicating real-world challenges. For evaluation, we designed physical environments with and without unforeseen indoor scenarios. This evaluation framework outputs statistical metrics, which we then use to conduct an extensive study on a deep RL method, namely the proximal policy optimization (PPO). The results provide valuable insights into the strengths and limitations of the method for mobile robot navigation. Our experiments demonstrate that the trained model from simulations can be deployed to the previously unseen physical world with a success rate of over 88%. The insights gained from our study can assist practitioners and researchers in selecting suitable RL approaches and training–testing settings for their specific robotic navigation tasks.